High oxidized low density lipoprotein, human

Catalog No:  TBP3

Lot No:                  

Quantity:    2.0mg(milligrams)Protein/Vial

Concentration:

Packaging:     High Oxidized LDL is membrane filtered and aseptically packaged in a solution containing phosphate-buffered saline at pH 7.4.

Preparation:    Thanbio Human LDL (Cat. No. TBP1) (which was purified to homogeneity via ultra centrifugation(1.019-1.063g/cc)) is extensively oxidized with Cu₂SO₄ (oxidant) in PBS at 37°C. Oxidation is terminated by adding excess EDTA-Na₂. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 3.0fold further than the native LDL. This product can produce potent oxidative stress and can be used to induce cell apoptosis/death(>50%) and produce cell injure model.

TBARS:    TBARS is determined colorimetrically by using Malondialdehyde as a standard.

                        Starting LDL    lot specific       nmoles of MDA/mg Protein

                        High Ox-LDL    lot specific     nmoles of MDA/mg Protein

Storage &                

Stability:    This product is stable for only 4 weeks after receipt when handled aseptically

             and stored at 2-8°C. NEVER FREEZE AND PROTECT FROM LIGHT.

*Special Note:    After prolonged storage, some precipitate may be observed. This is normal for this product. Clarify out the aggregates by spinning in a microfuge for 2 minutes.

Biological        Sample lots of Thanbio Oxidized LDL are evaluated for receptor binding to

Activity:           peritoneal macrophages in conjunction with our DiI-Ox-LDL and Ox-LDL

                                *Preparations of High Oxidized LDL are fairly unstable; Plan your experiments in advance and use fresh materials.

    FOR RESEARCH USE ONLY

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